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Archives



September 22, 1999 Update

Prepared by Dr. Michael Huerkamp


Where do we stand?


Since July, we have tested 60 sentinel mice and found 13 seropositive for MPV. All of these mice were derived from the same vendor and 11 were received on the same date from the same site. In no case have we found MPV DNA in any of these mice when assessed by PCR. The detection of positive sentinels has, however, resulted in the imposition of quarantine isolation on five colony rooms. Encouragingly, testing 354 mice from our colonies, with another 20 pending, has shown no evidence of MPV. These findings suggest, but do not prove, that we may have acquired seropositive mice from the vendor or that the mice may have become infected with MPV either during or shortly after shipment. Transportation of rodents, particularly via air, where they may be mixed with other cargo and potentially exposed to other rodents in cargo holding areas during layovers, carries with it an estimated risk of infection of 1.25% (Lab Anim Sci 48(5): 438-47, 1998). The vendor has been informed of our suspicions and asked to redouble efforts to assess for MPV.




What does the immediate future hold?


As mentioned in my last update, the sentinel program has been beefed-up by quadrupling the number of sentinels per rack so as to provide indirect exposure to more mice quickly. This increase in surveillance should provide valuable information relevant to MPV during the September and October sentinel assessments and will permit us to discontinue the more expensive practice of testing large numbers of mice selected from each room. It should also enable us to determine whether we must continue to quarantine colonies that have been isolated since the first positive sentinels were reported. This situation has also afforded DAR the opportunity to review our current quarantine and sentinel program practices with our faculty advisory committee and to refine our practices to better and more safely and effectively serve all rodent users. While our findings are encouraging, this is no time to let up. Renewed vigilance and continued adherence to preventive medicine practices as described in the main "Mouse Parvovirus Outbreak" posting remain paramount.