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DAR To Charge for Overcrowded Rodent Cage Notifications


In the past, the DAR would provide a free courtesy notification of overcrowded cages only taking action 48 hours later to redistribute mice while also charging a per cage "intervention fee", currently $20, but only upon taking action. The DAR has changed this practice to assess a "notification fee" for each cage of breeding mice that is overcrowded by definition and at the time that the notification of overcrowding is sent. Thus, there will be no cost-free period to correct cage overcrowding. Charges will accrue at the time of notification.


This change becomes effective June 1, 2012.


The reason for this change is to ensure that laboratories responsibly managing their mouse colonies are not financially burdened via the per diems in subsidizing the operation of breeding colonies where the colony managers are less than attentive. In a significant number of instances and on a regular basis the DAR has been "gamed" by languid, but cagey, mouse colony managers who developed the habit of awaiting notifications and then interceding to redistribute breeding mice and pups just at the cusp of DAR intervention and fee levying. This resulted in increased DAR efforts and costs that were not directly recouped and thus were folded into the per diem rates where these casual breeding colony management practices were and have been subsidized by all investigators.


All DAR managed facilities where mice are maintained.

Regulatory Basis

The IACUC developed, passed and posted a policy on the subject of "Mouse Overcrowded Cage Policy". This policy is based upon mouse population density and space requirements provided in the newly-issued 8th edition of the "Guide for the Care and Use of Laboratory Animals" and clarifying guidance from the NIH and AAALAC International.

Mouse overcrowded cages at Emory generally distribute along two lines:

  • (1) Pups in excessive numbers either from (a) failing to wean litters in a timely fashion such that numerous animals of multiple ages accumulate in a cage with younger mice often being trampled and out-competed for milk and (b) failing to split harems (e.g., trios) at appropriate times resulting in an excessive pup critical mass. These situations will be the emphasis of the DAR and the notification charges.
  • (2) simply housing too many adult mice per cage (e.g., 6-7 adult mice in the cage when only 5 are permitted by policy) - this aspect will be managed by the IACUC and not by DAR.

Exceptions to this policy and to these conditions are possible, but must be specifically approved by the IACUC, defined in the protocol, and with a visual designation on applicable mouse cages.

Helpful Advice

To avoid falling prey to DAR notification charges and to remain in compliance with the aforementioned IACUC policy, it is recommended that the following be reviewed with research staff, specifically those managing breeding colonies, at a laboratory meeting or other like forum:

  • Breeding colonies should be checked daily by persons responsible for managing production for several reasons, but in this case to prevent new litters from being vulnerable to the dominant effects of older pups from pre-existing litters.
  • Mouse pups must be weaned between 19 and 24 days of age.
  • Where mice in production are maintained as trios continuously, pup density must be reduced to a maximum of 12 pups per cage where one or more pups is at least 14 days of age.

Prepared by: Michael J. Huerkamp, DVM, DACLAM
Director, Division of Animal Resources and Attending Veterinarian
April 25, 2012