Contact Information


  • Division Secretary


    General Questions
    Phone: 404.727.7423
    Fax: 404.727.3212
    Email Us

  • Customer Service


    Billing
    Phone: 404.727.8395
    Fax: 404.727.8762
    Email Us

  • Animal Orders & Transfers
    Phone: 404.727.7426
    Fax: 404.727.8762
    Email Us

    Speed Types Add/Modify
    Phone: 404.727.7426
    Phone: 404.727.3210
    Fax: 404.727.8762
    Email Us

  • Facility Access


    Normal Business Hours

    (Monday thru Friday, 8AM - 5PM)


    Phone: 404.727.8991
    Email Us


  • After Normal Business Hours

    (Weekends, Holidays & After Hours: 5PM - 8AM)


    Phone: 404.727.6111
  • Animal Care & Husbandry


    Normal Business Hours

    (Monday thru Friday, 8AM - 5PM)


    Phone: 404.727.2955

  • Veterinary Medical Needs


    Normal Business Hours

    (Monday thru Friday, 8AM - 5PM)


    Phone: 404.727.3248

  • After Normal Business Hours

    (Weekends, Holidays & After Hours: 5PM - 8AM)


    Phone: 404.727.6111


Postal Mailing Address


  • 615 Michael Street
    Whitehead Biomedical Research Bldg
    Suite G-02
    Atlanta, GA 30322
    MAILSTOP: 1941-001-1AA

  • Business Hours

  • Monday - Friday
    8:00AM - 5:00PM





Emory Rodent Colony Health Surveillance and Maintenance Program



Background


Emory University has been continuously accredited by AAALAC international since 1992. Rodents entering into colonies maintained at Emory University Division of Animal Resources DAR may originate from one of three sources:

  1. 1. Emory DAR's List of Approved Vendors
  2. 2. DAR Rodent Quarantine Program
  3. 3. Mouse Transgenic and Gene Targeting Core (TMF)


Rodents are verified to be free from a defined set of adventitious pathogens upon entry into a colony, and policies are in place to help maintain this ‘Specific Pathogen Free’ (SPF) status. The primary objective of the health surveillance program is to monitor colonies for the presence of agents that would compromise the SPF status so that appropriate management steps can be taken should they be found.



Primary Rodent Housing Locations

High Level SPF Facility

Conventional SPF Facilities

Health Sciences Research Building (HSRB)

Whitehead Biomedical Research Building (WBRB)

 

O. Wayne Rollins Research Building (RRB)

 

Woodruff Memorial Research Building (WMRB)

 

Pediatrics Facility (Peds)

 

Clinic B 3rd Floor  (B3 3rd)

 

Clinic B Tunnel Level (B-Tun)

 

Peavine Kennel (PEA)

General Husbandry Practices for Rodents in DAR


Rodent housing rooms, except under special conditions, are under negative relative air pressure with respect to adjacent corridors. Colony animal cage bottoms are changed out weekly on static microisolator cages and every two weeks on ventilated cage systems. Corncob bedding and a cotton fiber nest material are the default for most mouse strains, with exceptions for specific strains or based on IACUC protocol requirements. Rodents are fed irradiated diets and water sanitation varies depending on building. Water bottles, if present, are replaced weekly and food is added during each cage change. Wire bar lids and food hoppers are replaced when visibly soiled, when a cage is depopulated, or at least every 6 months. Sterile housing is available for mice which includes an autoclaved water bottle and a complete autoclaved cage change weekly.

Rats may be housed in solid bottom cages without filter tops, in static filter top cages, in ventilated caging systems, or in suspended stainless steel cages. Cages, food, water and bedding for immunocompetent rats typically are not autoclaved prior to use, but cages are sanitized at 180°F. Personnel entering rooms must wear dedicated gowns and gloves when handling rats and use a chlorine dioxide disinfectant in the animal facility.


Rodent Colony Health Reports


A customized rodent colony health report can be requested online through DAR Quality Assurance and Diagnostic Laboratory here:
Emory Rodent Colony Health History Request. Requested reports will be provided to the requestor within 48 hours during business hours.


Biological Materials Screening Policy


All biological materials administered to rodents in Emory colonies must be screened for adventitious pathogens in accordance with DAR policy, which can be viewed here:
Biological Materials Screening Policy

 


Rodent Colony Health Surveillance Program Overview


Live animals are no longer used for health surveillance in the DAR rodent colonies.  One empty sanitized and autoclaved colony cage is placed per each side of every populated ventilated rack and one cage per static rack for an average testing ration of 1:70. During cage change, dirty bedding is transferred from populated colony cages to the empty health surveillance cage located on the rack. No more than 14 cages are sampled per cage change. Dirty bedding transfer is continued for 3 months (90 days). Colony surveillance cages are then collected from their housing rooms and are replaced with a new cage to continue surveillance. Collected colony surveillance cages are then processed for testing by collecting multiple sample types including surface swabs, fecal pellets and filter media. These samples are pooled at the room level accordingly and submitted to third-party commercial laboratories for PCR diagnostic analysis. Conventional SPF housing locations are minimally tested for the agents and at the frequency shown in Tables 1 and 3 (mice and rats, respectively). The additional agents monitored and frequency tested in high level SPF rooms are shown in Table 2. Note that some buildings have specialized rooms that necessitate testing in addition to that shown in the tables, and health reports for those rooms will indicate any additional agents monitored. Please request a health report for further details.



Table 1. Agents monitored in conventional SPF mouse housing*


Organism

Test Method

Frequency

Status

Sendai Virus (SEND)

PCR

Quarterly

Excluded

Mouse Hepatitis Virus (MHV)

PCR

Quarterly

Excluded

Mouse Minute Virus (MVM)

PCR

Quarterly

Excluded

Mouse Parvovirus (MPV-1)

PCR

Quarterly

Excluded

Mouse Parvovirus (MPV-2)

PCR

Quarterly

Excluded

GD VII Virus (Theiler’s)

PCR

Quarterly

Excluded

Mouse Rotavirus-A (EDIM)

PCR

Quarterly

Excluded

Lymphocytic Choriomeningitis (LCMV)

PCR

Quarterly

Excluded

Polyoma Virus (POLY)

PCR

Quarterly

Excluded

Mouse cytomegalovirus (MCMV)

PCR

Quarterly

Excluded

Mouse Poxvirus (Ectromelia)

PCR

Annually

Excluded

K Virus (K)

PCR

Annually

Excluded

Mouse adenovirus (MAV 1&2)

PCR

Annually

Excluded

Mycoplasma pulmonis (MPUL)

PCR

Annually

Excluded

Pneumonia virus of mice (PVM)

PCR

Annually

Excluded

Reovirus (REO)

PCR

Annually

Excluded

Hantavirus Hantaan (HANT)

PCR

Annually

Excluded

Lactate Dehydrogenase Elevating Virus (LDV)

PCR

Annually

Excluded

Mouse Thymic Virus (MTLV)

PCR

Annually

Excluded

 Filobacterium rodentium (CAR Bacillus)

PCR

Annually

Excluded

Encephalitozoon cuniculi (E. cuniculi)

PCR

Annually

Excluded

Murine Fur Mite (Myobia, Myocoptes, Radfordia)

PCR

Quarterly

Varies

Pinworms (Aspiculuris, Syphacia)

PCR

Quarterly

Excluded

Corynebacterium bovis *Sterile Housing Only*

PCR

Quarterly

Monitored

Mouse norovirus (MNV)

PCR

Annually

Monitored

 



Table 2. Status for higher SPF housing: Status same as listed in Table 1 unless specified below.*

 

Organism

Test Method

Frequency

Status

Helicobacter spp.

PCR

Quarterly

Excluded

Murine Fur Mite (Myobia, Myocoptes, Radfordia)

PCR

Quarterly

Excluded

Mouse norovirus (MNV)

PCR

Quarterly

Excluded

Corynebacterium bovis  *Sterile Housing Only*

PCR

Quarterly

Varies

Bordetella bronchiseptica

PCR

Annually

Excluded

Corynebacterium kutscheri

PCR

Annually

Excluded

Citrobacter rodentium

PCR

Annually

Excluded

Salmonella spp.

PCR

Annually

Excluded

Streptobacillus moniliformis

PCR

Annually

Excluded

 



Table 3. Agents monitored in conventional SPF rat housing*

 

Organism

Test Method

Frequency

Status

Pneumonia Virus of Mice (PVM)

PCR

Annually

Excluded

Sialodacryoadenitis Virus (SDAV

PCR

Annually

Excluded

Rat Theilovirus (RTV)

PCR

Annually

Excluded

Mouse Adenovirus 1 (MAdV-1)

PCR

Annually

Excluded

Mouse Adenovirus 1 (MAdV-2)

PCR

Annually

Excluded

Reovirus (REO-3)

PCR

Annually

Excluded

Sendai Virus (SV)

PCR

Annually

Excluded

Rat Parvovirus (RPV)

PCR

Quarterly

Excluded

Rat Minute Virus (RMV)

PCR

Quarterly

Excluded

Kilham's Rat Virus (KRV)

PCR

Quarterly

Excluded

Toolan's H-1 Virus (H-1)

PCR

Quarterly

Excluded

Mycoplasma pulmonis

PCR

Annually

Excluded

Pasteurella pneumotropica

PCR

Annually

Monitored

Filobacterium rodentium (CAR Bacillus)

PCR

Annually

Monitored

Pneumocystis spp.

PCR

Annually

Monitored

Streptobacillus moniliformis

PCR

Annually

Monitored

Radfordia ensifera

PCR

Quarterly

Excluded

Pinworms

PCR

Quarterly

Excluded


*Definitions:

Excluded: Actions are taken if a positive result is found. Actions include researcher notification, room quarantine and additional testing of colony animals to confirm the positive and its source. The specific processes depend on the agent and circumstance.

Monitored: No definitive action is taken if a positive is noted.

Varies: Agent is either excluded or monitored based on the status of the room. Please note that we have an initiative for campus wide eradication of fur mites that is in progress.